ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the susceptibility of C6 glioma cells to Myxoma virus and the killing effect of Myxoma virus to the C6 glioma cells in vitro.</p><p><b>METHODS</b>C6 glioma cells were infected with myxoma virus, used death virus as the negative control, 5-FU as the positive control, DEMD as blank control. The number of living cells were counted every 24 h, and Western-Blot method, inverted microscope and MTT assay were applicated to observe the cell morphology and survival rate in each group.</p><p><b>RESULTS</b>The cell number were decreased rapidly in virus effected group and 5-FU group, with significant differences to the negative and blank control groups. And cells in virus effected group appeared cytopathic effect.</p><p><b>CONCLUSIONS</b>C6 glioma cells were susceptible to myxoma virus and myxoma virus had killing effect to C6 glioma cells in vitro.</p>
Subject(s)
Humans , Cell Line, Tumor , Glioma , Therapeutics , Myxoma virus , Oncolytic Virotherapy , Proto-Oncogene Proteins c-akt , PhysiologyABSTRACT
We found seven tag sequence with high homology in dbEST by using human musclin gene, and got its cDNA sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by RT-PCR, encoding 132 amino acid residue protein. The new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pGEX-4T-1, and the recombinant was transformed into E. coli BL21. Induced by IPTG, the fusion protein GST-musclin, a 38.59 kD protein was successfully expressed in E. coli BL21 and identified by Western blotting.
Subject(s)
Animals , Humans , Mice , Rats , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA, Complementary , Genetics , Escherichia coli , Genetics , Metabolism , Molecular Sequence Data , Muscle Proteins , Genetics , Muscle, Skeletal , Metabolism , Open Reading Frames , Genetics , Recombinant Fusion Proteins , Genetics , Swine , Genetics , Transcription Factors , GeneticsABSTRACT
Alternariol monomethyl ether(AME) was isolated, purified and crystallized from the extracts of the corn inoculated with Alternaria alternata which was separated from the contaminated rice in the high incidence area of esophageal cancer, Lixian County. AME is one of the major metabolic products of A. alternata. In this paper, the mutagenic potency of AME was detected with the V_(79) Chinese hamster cells. The experimental results showed that with or without rat liver microsomes (S-9), AME could induce 6-Thioguanine resistant (6-TG~r) mutation in V_(79) cells and their dose-effect curves were similar. The mutagenicity of AME without S-9 was stronger than that of AME with S-9. So it is considered that AME is a direct mutagen which doesn' t need to be activited metabolically by S-9. AME may play an important role in the etiology of esophageal cancer.